CpG-free Genes

Synthetic CpG-free Genes

Bacterial DNA is rich in unmethylated cytosine-phosphate-guanine (CpG) sites, unlike mammalian DNA, in which most CpG sites are methylated (specifically, at the 5’-position of the cytosine). Unmethylated CpGs, in specific sequence contexts, activates the vertebrate immune system. Genes with these sequences are recognized as foreign DNA by the vertebrate host, leading to a progressive decline in their expression.

Many non-mammalian (i.e. bacterial) genes are widely used as reporter or suicide genes in molecular and cellular studies. To circumvent this limitation, InvivoGen has synthesized new alleles of these genes completely devoid of CpG dinucleotides. These synthetic CpG-free genes display higher activity and lower immunogenicity than their wild-type counterparts.

InvivoGen is an expert in developing CpG-Free genes and now offers a custom CpG-Free gene service. Send us the sequence of your gene of interest, and our specialists will design, synthesize, and clone into an expression vector, a CpG-Free allele of this gene. We guarantee that the sequence of the synthetic gene will 100% match the designed sequence.

Products

CpG-free Reporter genes
CpG-free GFP::Sh		Synthetic reporter / resistance fusion gene in expression plasmid
CpG-free GFP::Bsr		Synthetic reporter / resistance fusion gene in expression plasmid
CpG-free LacZ		Synthetic LacZ reporter gene in expression plasmid
CpG-free LacZnls		Synthetic LacZ reporter gene with SV40 nuclear localization signal in expression plasmid
CpG-free LacZ::Sh		Synthetic reporter / resistance fusion gene in expression plasmid
CpG-free Luc::Sh		Synthetic reporter / resistance fusion gene in expression plasmid
CpG-free SEAP		Synthetic SEAP reporter gene in expression plasmid
CpG-free Cytotoxic genes
CpG-free Fcy::fur		Synthetic cytotoxic CD::UPRT fusion gene in expression plasmid
CpG-free HSV1-tk		Synthetic cytotoxic viral TK gene in expression plasmid
CpG-free HSV1-tk::Sh		Synthetic cytotoxic / resistance fusion gene in expression plasmid
CpG-free & Low-CpG Resistance genes
CpG-free hph		Synthetic hygromycin resistance
CpG-free neo		Synthetic neomycin resistance
CpG-free Sh ble		Synthetic Zeocin resistance
Low-CpG bsr		Synthetic blasticidin resistance
Low-CpG pac		Synthetic puromycin resistance