Custom Cloning
| Custom-made pSELECT | Unit size | Cat. code | € | $ | £ | Order |
|---|---|---|---|---|---|---|
|
Gene subcloning
|
20 ug | p-custom-select | From 800€ | From $1100 | From £600 | Ask for a quote |
| Custom-made pDRIVE | Unit size | Cat. code | € | $ | £ | Order |
|---|---|---|---|---|---|---|
|
Promoter assembly and subcloning
|
20 ug | p-custom-drive | From 800€ | From $1100 | From £600 | Ask for a quote |
Customized plasmid construction suited to your needs
InvivoGen is at the leading edge of research and development in vector construction, gene delivery, and gene expression systems. With more than 20 years’ experience in cloning, our specialists offer a choice of custom cloning services at very competitive rates with a short turn-around time. Our expert team works closely with you to provide customized plasmids that are designed for your specific research needs.
Services
- Custom-made pSELECT : Gene subcloning
- Custom-made pDRIVE : Promoter assembly and subcloning
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Description
• Gene subcloning : Custom-made pSELECT
InvivoGen offers a large collection of genes provided in pUNO1, an expression plasmid selectable with Blasticidin. If the use of this antibiotic does not suit your needs, our experts can subclone any of our genes into a pSELECT plasmid carrying the selectable marker of your choice : hygromycin, G418/Geneticin, puromycin, Zeocin or GFP::Zeocin.
• Promoter assembly and subcloning : Custom-made pDRIVE
You can create your own composite promoter by combining the enhancer, core promoter and 5’UTR chosen from InvivoGen’s extensive collection (listed below). We will assemble this composite promoter in a pDRIVE plasmid harboring a reporter gene (SEAP, Lucia luciferase or GFP). Additionally, you can choose a gene of interest from our library. The pDRIVE plasmid is provided with a selectable marker Zeocin®.
Contents
Custom-made pSELECT and pDRIVE plasmids are provided as 20 µg of lyophilized DNA.
Products are shipped at room temperature.
Upon receipt, products should be stored at -20°C.
Custom-Made pDRIVE List
| ENHANCERS | |||
| Enhancer | Name | Origin | Specificity |
| AldA | Aldolase A | Human | Ubiquitous |
| AFP | Alpha-Fetoprotein | Human | AFP-positive cells |
| CEA | Carcinoembryonic antigen | Human | CEA-positive cells |
| hCMV | Human Cytomegalovirus | Viral | Ubiquitous |
| mCMV | Mouse Cytomegalovirus | Viral | Ubiquitous |
| P2 | Adipocyte P2 | Mouse | Adipose tissue |
| PSA | Prostate specific antigen | Human | PSA-positive cells |
| SV40 | Simian virus | Viral | Ubiquitous |
| Tie2 | Angiopoietin receptor | Mouse | Vascular endothelial cells |
| Tyr | Tyrosinase | Mouse | Melanocytes and melanoma |
| CORE PROMOTERS | |||
| Ubiquitous Promoter | Name | Origin | Specificity |
| Beta-Act | Beta-Actin | Human | Ubiquitous |
| hCMV | Human Cytomegalovirus | Viral | Ubiquitous |
| hEF-1alpha | Elongation Factor 1 | Human | Ubiquitous |
| rEF-1alpha | Elongation Factor 1 | Rat | Ubiquitous |
| chEF-1alpha | Elongation Factor 1 | Chimpanzee | Ubiquitous |
| FerH | Ferritin H | Human | Ubiquitous |
| FerL | Ferritin L | Human | Ubiquitous |
| Tissue-Specific Promoter | Name | Origin | Specificity |
| bAlb | Albumin | Bovin | Liver |
| hAlb | Albumin | Human | Liver |
| CD43 | Leukosialin | Human | Leukocytes and platelets |
| CD45 | Leukocyte Common Antigen | Human | Hematopoietic cells |
| NSE | Neuron-Specific Enolase | Rat | Mature neurons |
| Tumor-Specific Promoter | Name | Origin | Specificity |
| AFP | Alpha-Fetoprotein | Human | Hepatocellular carcinoma |
| CEA | Carcinoembryonic Antigen | Human | Epithelial cancers |
| PSA | Prostate Specific Antigen | Human | Prostate cancers |
| Tyr | Tyrosinase | Mouse | Melanoma |
| 5' UNTRANSLATED REGIONS | |||
| 5' UTR | Name | Origin | Activity |
| AdTp | Adenotripartite | Viral | Enhances translation |
| mEF-1alpha | Elongation Factor 1 | Mouse | Increases mRNA translation |
| chEF-1alpha | Elongation Factor 1 | Chimpanzee | Increases mRNA translation |
| eIF4g | Eukaryotic initiation factor 4g | Human | Contains a putative IRES |
| HSP70 | Heat shock protein 70 | Human | Increases mRNA translation |
| NRF | NF-κB repressing factor | Mouse | Acts as a potent IRES |
| HTLV RU5’ | Human T-cell leukemia virus | Viral | Increases mRNA translation |