Custom Cloning Service

At InvivoGen, we ensure every custom plasmid meets the highest standards of quality and consistency through a comprehensive suite of quality control measures:

• Full-Length Sequencing - Complete plasmid verification using third-generation sequencing.
   
• Restriction Enzyme Digestion Analysis - Verification of key restriction sites via enzymatic digestion.
   
• Plasmid Integrity Assessment - Gel electrophoresis analysis confirming plasmid homogeneity (> 80% supercoiled).
   
• Purity Checks - Assessment of DNA purity using A260/280 and A260/230 absorbance ratios.
   
• Mycoplasma Testing (Optional) - Optional mycoplasma contamination testing is also available.
   
• Endotoxin Testing (Optional) - Our Gigaprep service uses an Endofree® kit to ensure minimal endotoxin levels. For sensitive applications, optional endotoxin quantification using the LAL assay.

Our plasmid collection supports diverse expression needs, from antibody production to controlled gene expression. Choose from vectors designed for in vitro or in vivo applications, including tagged proteins, inducible reporter systems, and transgenesis models.

Explore the options below and create a promoter tailored to your experimental needs:

Can’t find what you’re looking for? Contact us. We have customizable options for you.

Our streamlined process ensures a smooth and transparent experience from design to delivery.

1. Contact - Reach out to technical support to discuss your project needs, preferred plasmid backbone, desired promoter, downstream application, target sequence, and required quantity. Together, we will define the project scope, timeline, and finalize the service agreement.

2. Molecular Construction - Your gene(s) of interest are subcloned into the chosen/custom-designed plasmid backbone. Our team prepares the plasmid at the requested scale, ensuring rigorous adherence to your specifications.

3. Quality Control - Plasmid constructs undergo stringent quality checks, including sequence verification of the entire plasmid and integrity validation of the final preparation.

4. Product Delivery - You receive the complete plasmid map, full plasmid sequence, and the requested plasmid quantity. Typical project timelines range from 2 to 4 weeks, depending on project complexity and customization needs.

Illustrations on this webpage are created with BioRender.com.

The customized plasmids are supplied according to your specifications.

• Format: Choose between lyophilized (dry) or liquid plasmid DNA.
• Buffer: Liquid DNA can be provided in the concentration and buffer of your choice (e.g., PBS, water, or a custom formulation).
• Quantity: Standard deliveries include 20  µg, but we can accommodate requests up to 10  mg upon demand.

Products are shipped at room temperature.

Upon receipt, products should be stored at -20°C.