Zeocin®

Selection antibiotic for bacteria and mammalian cells – Endotoxin-tested

Zeocin® is a formulation of phleomycin D1 used for the selection of bacteria and eukaryotic cells, provided as a sterile, cell culture-tested solution or powder. This member of the bleomycin/phleomycin family is a water-soluble, copper-chelated glycopeptide antibiotic, produced by a proprietary strain of Streptomyces verticillus. Zeocin® causes cell death by intercalating into DNA and cleaving it. The action of Zeocin® is effective on most aerobic cells, such as bacteria, eukaryotic microorganisms, plant cells, and animal cells.

Resistance to Zeocin® is conferred by the Sh ble gene product from Streptoalloteichus hindustanus, which inactivates Zeocin® upon binding to the antibiotic [1-3]. Zeocin® is an effective antibiotic for the selection of bacteria and eukaryotic cells expressing the Sh ble resistance.

Due to its activity in both E. coli and mammalian cell lines, vectors can be designed that carry only one drug resistance marker for selection. Suggested concentrations are 50 - 400 µg/ml for selection in mammalian cells and 25 - 50 µg/ml for selection in E. coli.

Key features

• Produced in InvivoGen's facilities. InvivoGen is the sole producer of Zeocin®
• Each lot thoroughly tested to ensure lot-to-lot reproducibility
• Potency validated in Zeocin®-sensitive and Zeocin®-resistant mammalian cell lines
• Absence of long-term effects confirmed in Zeocin®-resistant cells
   

References:

1. Drocourt D. et al., 1990. Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucl. Acids. Res. 18: 4009.
2. Gatignol A. et al., 1988. FEBS Letters. 230: 171-5.
3. Dumas P. et al., 1994. Embo J. 242 (5) 595-601.