pFUSEN-Lucia-hG1e2Fc

Production of Lucia luciferase-tagged IgG1e2-Fc fusion protein

pFUSEN-Lucia-hG2Fc is a cloning plasmid for the generation of a Lucia luciferase-tagged engineered Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region fused to the N-terminus of a protein of interest.

Lucia luciferase is a secreted reporter protein with advantageous characteristics when associated with Fc-fusion proteins.

It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.

Human IgG1e2-Fc contains mutations in the site of interaction of IgG1 with FcRn that enhance the plasma half-life by increasing the affinity of IgG1 for FcRn.