Engineered Human Reporter HepG2 Liver Carcinoma Cells
HepG2 human hepatoma cells were originally isolated from the liver tissue of a patient with a well-differentiated hepatocellular carcinoma. This adherent-growing cell line has become a commonly used cell model to study cellular responses to infection with hepatotropic pathogens. HepG2 cells are also used in biomedical research to investigate drug metabolism in the liver. Since they endogenously express a variety of antioxidant and xenobiotic metabolizing enzymes, HepG2 cells are a suitable tool for detecting environmental pollutants and dietary-derived cytotoxic and genotoxic compounds.
Therefore, HepG2 cells are a great tool to:
- study hepatocarcinogenesis and liver diseases
- examine hepatotropic pathogen infections
- investigate drug metabolism and cell signaling pathways
- screen for gut microbiota-derived or xenobiotic ligands
InvivoGen provides HepG2-derived cell lines designed for the study of the NF-κB and IRF pathways or the aryl hydrocarbon receptor (AhR) pathway in a liver cell line.
HepG2-Dual™ reporter cells feature two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase. As a result, these cells allow the simultaneous study of the NF-κB and/or the IRF pathways, by assessing the activity of SEAP or the secreted Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
HepG2-Lucia™ AhR cells express the aryl hydrocarbon receptor (AhR) as well as the inducible reporter gene for Lucia luciferase. As a result, these cells allow the study of the AhR genomic signaling pathway, by assessing the activity of secreted Lucia luciferase. The reporter protein is readily measurable in the cell culture supernatant when using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
All our cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.
Key Features
- Stable expression of one or two reporter systems
- Quantifiable responses upon stimulation with xenobiotics, synthetic dietary-derived, or endogenous AhR agonists
- Functionally tested and guaranteed mycoplasma-free
Fields of Interest
- NF-κB / IRF pathways
- Aryl hydrocarbon receptor (AhR) pathway
