InvivoGen provides reporter cell lines derived from different human cell types designed for the study of regulated cell death, in particular ferroptosis, necroptosis, pyroptosis and oxeiptosis.
Regulated cell death reporter cells comprise:
• HT1080-HMGB1-Lucia™ reporter cells, for the study of ferroptosis
These cells, derived from human HT-1080 fibrosarcoma cells, stably express the alarmin High-Mobility-Group-Protein B1 (HMGB1) fused to the secreted Lucia luciferase reporter (HMGB1::Lucia-luciferase). Upon ferroptotis activation, this fusion protein is released; its levels can be determined by measuring the light signal produced.
• THP1-HMGB1-Lucia™ reporter cells, for the study of necroptosis and pyroptosis
These cells are derived from the human THP-1 monocytic cell line. They express the HMGB1::Lucia-luciferase fusion reporter protein which is released upon necroptosis or pyroptosis. The release of the reporter protein can be assessed by mesuring the levels of Lucia luciferase.
• HEK-Lucia-Star™ ARE reporter cells, for the study of oxeiptosis
These cells, derived from human embryonic kidney (HEK) 293 cells, feature an ARE-inducible Lucia reporter gene. Expression of Lucia is proportional to the oxidative stress response and thus oxeiptosis.
InvivoGen also offers a selection of inhibitors, known to modulate regulated cell death.