Bioluminescent ELISA kits for human and mouse IFN-α/β detection
InvivoGen’s LumiKine™ Xpress 2.0 kits offer a rapid, sensitive bioluminescent ELISA format for type I interferon (IFN-α/β) detection in human and mouse samples. They have been optimized to reduce hands-on time while retaining high sensitivity and reliability, allowing cytokine detection in various biological samples (cell culture supernatant, serum, and plasma).
LumiKine™ Xpress 2.0 kits are sandwich ELISAs requiring only a single 2-hour incubation in which the cytokine binds to both an immobilized capture antibody, and to a detection antibody conjugated to Lucia® luciferase.
The concentration of the cytokine in the various samples is determined using QUANTI-Luc™ 4 Reagent, a Lucia® luciferase detection reagent. The bioluminescent signal is emitted instantly (saving even more time) and is readily assessed by luminometry.
Features of the LumiKine™ Xpress 2.0 kits:
- Simple: Easy 4-step protocol
- Fast: Single 2-hour incubation and immediate detection
- Highly sensitive: Limit of detection <10 pg/ml
- Robust and reliable: Consistent and reproducible results
Applications
- Immunomodulatory drug screening
- Antiviral therapeutic development
Safety profiling
