LumiKine™ Xpress mIFN-β 2.0

LumiKine™ Xpress mIFN-β 2.0

Rapid bioluminescent murine IFN-β ELISA kit

LumiKine™ Xpress mIFN-β 2.0 is InvivoGen’s second-generation bioluminescent murine interferon-beta (mIFN-β) ELISA, using a Lucia luciferase-conjugated detection antibody. LumiKine™ Xpress mIFN-β 2.0 is an optimized ‘sandwich’ ELISA kit designed to rapidly and reliably measure the concentration of mIFN-β in various biological samples (cell culture supernatant, serum, and plasma).

After a single 2-hour incubation, the detection of mIFN-β is instantaneously determined using QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent. The bioluminescent signal is readily assessed by luminometry.

Features of LumiKine™ Xpress mIFN-β 2.0:

• Single incubation: 2 hours
• Limit of Detection: 5 pg/ml
• Specificity: Murine IFN-β
• Optimized antibody pairing: The capture and detection antibodies bind to different epitopes of the cytokine
• No cross-reactivity with human (h)IFN-α2, hIFN-β or mIFN-α2

LumiKine™ Xpress mIFN-β 2.0 is provided with a standard recombinant mIFN-β produced in mammalian cells, to ensure the most accurate and sensitive calibration for the measurement of unknown levels of mIFN-β.

Figures

Figure 1: (A) A 7-point standard curve (beginning at 1000 pg/ml) was generated using a two-fold serial dilution of standard mIFN-β. (B) Wild type (WT) and STING knock out (KO-STING) mice were injected intraperitoneally with CL592 (2 x 100 mg/kg), a STING agonist. After 4 hours, serum was isolated and diluted 1/200 using DMEM and 10% heat-inactivated (HI)-FBS. The concentration of mIFN-β in both samples was quantified using LumiKine™ Xpress mIFN-β 2.0.

Specifications

Incubation time: 2 hours (after plate preparation)

Limit of detection: 5 pg/ml

Specificity: No cross-reactivity with mIFN-α2, human (h)IFN-α2 or hIFN‑β

Target: Natural and recombinant murine IFN-β

Standard: HEK293-expressed murine IFN-β

Measurement: Bioluminescent ELISA - relative light units (RLUs)

Sample size and type: 50 µl of cell culture supernatant, serum, and plasma

Quality control:

• The sensitivity of LumiKine™ Xpress mIFN-β 2.0 has been validated.

Contents

LumiKine Xpress™ mIFN-β 2.0 contains:

• 150 µg lyophilized mIFN-β capture antibody
• 5 µg lyophilized Lucia-conjugated detection antibody
• 100 ng lyophilized mIFN-β standard (HEK293-expressed recombinant cytokine)
• 2 tubes of QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent (sufficient to prepare 50 ml)
• 5 white flat-bottom MaxiSorp® 96-well plates with 5 plate sealers

Products are shipped at room temperature.

Upon receipt, store at -20°C.