Frequently asked questions

The cost of a customized cell line depends on multiple factors, including the complexity of genetic modifications, choice of the host cell line, licensing requirements, and additional validation steps. Just contact us, share your requirements, and we’ll provide a personalized quote.

The timeline varies depending on the complexity of the project and requests; it also depends on the cell line, growth characteristics, and culturing requirements. Typically, it takes our experts between 20 to 25 weeks, including cloning, selection, validation steps, reporting, and delivery.

Yes, we offer expedited development options to shorten the timeline where possible. Contact us to discuss your specific requirements.

Absolutely. You can choose multiple clones. For your convenience and scalability, we offer cell banking services, allowing for the production and delivery of multiple vials of your chosen clone(s) upon request.

Absolutely. We perform thorough QC testing, including sterility, mycoplasma detection, stability (up to 10 passages), expression validation, and functional assays to ensure high-quality results.

We perform stability tests to guarantee stability for up to 10 passages.

Our tech support team can advise on the best host cell line based on your research application, whether you need an immune cell model, adherent cells, or suspension culture. We work with a wide range of mammalian cell lines, including HEK293, THP-1, Jurkat, A549, Raji, RAW-264, and B16 cell lines.

No. To streamline your workflow we include complimentary samples of essential assay reagents, such as suitable selection antibiotics and reporter detection reagents with your final product delivery.

The cell-based data provides a more holistic understanding of the biological activity and the resulting cellular responses. This allows more informed decisions to be made about which compounds should enter therapeutic development.

Absolutely. We offer custom screening options for specialized research requirements. Whether you need unique PRR pathways or specific concentrations, our team can tailor the assay to meet your study’s needs.

Yes, of course! Our PRR Screening Service is designed to assess the potential activity of your compounds or extracts on various PRRs, providing valuable insights into their immunomodulatory effects.

Absolutely. InvivoGen offers many reporter cell lines suitable for screening lead compounds. From cytokine signaling to immune checkpoints, our team can tailor the assay to meet your study’s needs.

The price depends on many factors, such as the screening level, number of compounds and cell lines, concentration ranges, and grade of customization. The indicated prices (see above) cover:

- Compound Profiling (Level 1): 1 compound at 1 concentration on our TLR panel (7 cell lines) + control cell line.

​- Compound Dose Response (Level 2): 1 compound at 5 concentrations on 1 PRR cell line + control cell line.

For more tailored screening options, contact us, share your requirements, and we’ll provide a personalized quote.

In our experience, a minimum of 7 concentrations be tested with a series of 1:3 dilutions to sufficiently calculate an EC50 or IC50.

With over 25 years of expertise, InvivoGen offers robust, sensitive assays and customizable options. Our reliable data analysis, confidentiality, and commitment to quality make us a trusted choice in immunology research services.

PRR profiling is critical in identifying compounds that influence immune responses, aiding in adjuvant and drug discovery for conditions like cancer, autoimmunity, and infectious diseases. This process accelerates early-stage drug development by providing reliable data on immune pathway modulation.

The cost depends on project complexity, plasmid design, backbone selection, sequence, and final DNA quantity. Just contact us with your project details for a personalized quote.

Project timelines vary depending on sequence complexity and requested quantities. Typically, your custom plasmid will be ready within 2 to 4 weeks from order confirmation.

Yes. Plasmids can be delivered either lyophilized/dry or in liquid format, in the concentration and buffer of your choice (e.g., PBS, TE buffer, nuclease-free water, or custom buffer).

Standard production range from 20 µg to 10 mg of plasmid DNA. Upon request, we can supply higher quantities to meet your experimental needs.

Yes. We provide full sequence verification of the entire plasmid using third-generation sequencing method, ensuring complete accuracy. A detailed plasmid map and the full verified sequence are provided upon delivery.

Every construct undergoes stringent quality control, including sequence confirmation, purity assessment, and integrity validation prior to shipment.

Absolutely. Our technical experts can assist you in choosing the optimal plasmid backbone and guide element selection based on your downstream application. Just reach out with your project details.

You will receive complete results as optical density (OD) values at 650 nm in an Excel file, along with the EC50 and/or IC50 for easier interpretation. A summary report with key findings and assay conditions is also provided.

In our experience, a minimum of 7 concentrations are tested with a series of 1:3 dilutions to sufficiently calculate an EC50 or IC50.

The price depends on many factors, such as the screening level, number of compounds and cell lines, concentration ranges, and grade of customization. The indicated prices (see above) cover:

• Cytokine Agonist Screening: 1 test compound at 11 (+ non-induced) concentrations on one chosen cell line. Negative control cell line and benchmark agonist included if available.
• Cytokine Antagonist Screening: 1 test compound at 11 concentrations on one chosen cell line and a chosen benchmark agonist at 3 concentrations. Negative control cell line and benchmark  neutralizing compound/antibody included if available.

For more tailored screening options, contact us, share your requirements, and we’ll provide a personalized quote.

All assays are performed by trained specialists using validated reporter cell lines and standardized protocols. Each condition is run in triplicate, and negative control cell lines, as well as benchmark agonists or antagonists are included whenever available to confirm assay performance.

Cell-based data provide a functional perspective on biological activity, capturing the downstream cellular responses that binding assays alone cannot reveal. Such information is essential for selecting compounds with true bioactivity for further therapeutic development, as ELISA or flow cytometry only demonstrate binding—not functional efficacy.

Absolutely. Customers may supply their own reference compound or antibodies for inclusion in the assay. We will handle all materials under strict confidentiality and incorporate them into the experimental design as requested.