TLR Reporter Cells

Engineered reporter cell lines to assess Toll-like receptor (TLR) signaling

InvivoGen provides a comprehensive set of human and mouse cell lines to monitor TLR activity using inducible SEAP (secreted embryonic alkaline phosphatase) and/or Lucia Luciferase reporter systems.

Three families of TLR cell lines are available:

Endogenous TLR expression

Cell lines that feature endogenous TLR gene expression in the mouse RAW-264.7 macrophage or human THP-1 monocyte background.

TLR overexpression (OE)

Cell lines that have been transfected to stably express a human or murine TLR gene in the human HEK293 embryonic kidney carcinoma or human THP-1 monocyte background.

TLR knockout (KO)

Cell lines that have been knocked out for the expression of a human or murine TLR gene in the human THP-1 monocyte or mouse RAW-264.7 macrophage background.

HEK 293 embryonic kidney cells
HEK-Blue™ TLR Cells		Human HEK293 embryonic kidney carcinoma - TLR overexpressing & NF-κB-SEAP Reporter Cells
HEK-Blue-Lucia™ TLR Cells		Human HEK293 embryonic kidney carcinoma - TLR overexpressing & NF-κB-SEAP and KI-[IL-8]Lucia Reporter Cells
HEK-Dual™ TLR Cells		Human HEK293 embryonic kidney carcinoma - TLR overexpressing, NF-κB-SEAP & IRF-Lucia Reporter Cells
RAW 264.7 macrophages
RAW‑Dual™ KO-TLR4 Cells		Mouse RAW264.7 Macrophages - TLR4 knockout & NF-κB-SEAP and IRF-Lucia Luciferase Reporter Cells
THP-1 monocytes
THP1-Dual™ hTLR3 Cells		Human THP-1 Monocytes - Human TLR3 overexpressing & NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ MD2-CD14-TLR4 Cells		Human THP-1 Monocytes - MD-2 and CD14 overexpression in NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ hTLR7 Cells		Human TLR7 overexpression or KO in NF-κB-SEAP and IRF-Lucia Reporter Monocytes
THP1-Dual™ hTLR8 Cells		Human TLR8 overexpression or KO in NF-κB-SEAP and IRF-Lucia Reporter Monocytes
THP1-Dual™ hTLR9 Cells		Human THP-1 Monocytes - Human TLR9 overexpressing & NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ KO-TLR2 Cells		Human THP-1 Monocytes - TLR2 knockout & NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ KO-TLR4 Cells		Human THP-1 Monocytes - TLR4 knockout & NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ MD2-CD14-KO-TLR4 Cells		Human THP-1 Monocytes - MD-2 and CD14 overexpression in NF-κB-SEAP and IRF-Lucia Reporter Cells
THP1-Dual™ hTLR7 KO-TLR8 Cells		Human TLR7 overexpression or KO in NF-κB-SEAP and IRF-Lucia Reporter Monocytes
THP1-Dual™ KO-TLR8 Cells		Human TLR8 overexpression or KO in NF-κB-SEAP and IRF-Lucia Reporter Monocytes