Engineered Human Reporter HT29 Colon Adenocarcinoma Cells
InvivoGen provides an HT29-derived reporter cell line. HT29 was originally isolated from a primary tumor obtained from a patient with colorectal adenocarcinoma. This human colorectal adenocarcinoma cell line is adherent with an epithelial morphology. HT29 cells express characteristics of mature intestinal cells and form a tight monolayer with an apical brush border. They exhibit similarities with enterocytes of the small intestine making them a valuable model for the study of colon cancers as well as studies focused on food digestion and gut microbiota.
InvivoGen has developed the HT29-Lucia™ AhR cell line, designed for the study of the aryl hydrocarbon receptor (AhR) genomic signaling induction. It features an inducible Lucia luciferase reporter construct allowing the study of the AhR genomic signaling pathway. The activity of Lucia luciferase is readily measurable in the cell culture supernatant when using the QUANTI-Luc™ 4 Lucia/Gaussia detection reagent.
This cell line is extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for this cell line, to minimize the need for optimization or troubleshooting by the end-user.
Key Features
- Stable expression of an AhR-inducible reporter gene
- Quantifiable responses upon stimulation with tryptophane byproducts
- Functionally tested and guaranteed mycoplasma-free
Fields of Interest
- Study of the AhR genomic signaling pathway
- Screening of AhR ligands
