QUANTI-Blue™ Solution
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Cat.code:
rep-qbs
- Documents
ABOUT
QUANTI-Blue™ Solution is a colorimetric enzyme assay developed to determine any alkaline phosphatase (AP) activity in a biological sample, such as cell culture supernatant. It has been optimized for use in 96-well plates (standard procedure) and in 1536-well plates (high-throughput screening procedure).
QUANTI-Blue™ Solution can be used to detect and quantify AP or SEAP activity. Secreted embryonic alkaline phosphatase (SEAP), a truncated form of placental AP, is a widely used reporter gene. SEAP is secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters. InvivoGen provides a wide collection of reporter cell lines with the SEAP reporter gene.
Features and advantages of QUANTI-Blue™ Solution:
- A convenient, easy, and rapid means to detect and quantify SEAP and AP activity
- Short hands-on-time: < 10 min
- Easy to prepare: just mix QB Reagent and QB Buffer with water
- Highly sensitive
- Wide dynamic range
- High saturation threshold
- Allows for high-throughput screening (HTS)
In the presence of AP or SEAP, the color of QUANTI-Blue™ changes from pink to purple/blue. The purple/blue color intensity reflects the activity of AP. The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
180 µl
Detection and quantification of SEAP activity
Standard or HTS
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:QUANTI-Blue™ Solution
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Cat code:rep-qbs
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Quantity:5 ml (100X)
- 5 x 1 ml of QB reagent
- 5 x 1 ml QB buffer
Sufficient to prepare QUANTI‑Blue™ Solution for 25 x 96-well plates or 20 x 1536-well plates.
Shipping & Storage
- Shipping method: Room temperature
- -20 °C
- Avoid repeated freeze-thaw cycles
- Protect from light
Storage:
Caution:
Details
QUANTI-Blue™ Standard procedure
1. Prepare QUANTI-Blue™ Solution as per the instructions on the data sheet. Dispense 180 μl of QUANTI-Blue™ Solution per well of 96-well plate.
2. Add 20 μl of culture supernatant from SEAP-expressing cells to each well.
3. After a 15 min to 6 hour-incubation at 37°C, measure optical density (OD) at 620-655 nm using a microplate reader
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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