HEK-Blue™ Detection - SEAP Detection Medium

HEK-Blue™ Detection is a cell culture medium developed to provide a fast and convenient method to monitor SEAP expression. Detection of SEAP occurs as the reporter protein is secreted by the cells grown in HEK-Blue™ Detection.

HEK-Blue™ Detection changes to a purple/blue color in the presence of alkaline phosphatase activity. SEAP is a widely used reporter gene. It is a truncated form of placental alkaline phosphatase, a GPI-anchored protein.

SEAP is secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters. It allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies. Using HEK-Blue™ Detection, SEAP expression can be observed visually, and unlike fluorescent or luminescent reporters can be easily quantified using a microplate reader or spectrophotometer.

HEK-Blue™ Detection is applicable to high-throughput screening.

Procedure:

1. Resuspend HEK-Blue™ Detection

2. Add SEAP-expressing cells

3. Transfer to multi-well plate

4. Incubate 6 to 24 hours at 37°C, 5% CO2

5. Assess SEAP levels