Blasticidin (10 mg/ml solution)
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Cat.code:
ant-bl-05
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ABOUT
Selection antibiotic for bacteria and mammalian cells – Endotoxin-tested
Blasticidin is a high-quality selective antibiotic for bacteria and eukaryotic cells, provided as a sterile, cell culture-tested solution or powder. Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery, in both prokaryotic and eukaryotic cells.
Resistance to blasticidin is conferred by various resistance genes: bsr from Bacillus cereus encoding a blasticidin deaminase [1], bls from Streptoverticillum sp encoding a blasticidin acetyltransferase [2], BSD from Aspergillus terreus encoding a blasticidin deaminase [3].
Since blasticidin is active in both bacteria and mammalian cell lines, vectors can be designed that carry only one drug resistance marker for selection. Selection is usually achieved within 7 days, with recommended working concentrations of 1-10 μg/ml for selection in mammalian cells and 25-100 μg/ml for selection in E. coli.
Key features
- Produced in InvivoGen's facilities
- Each lot is thoroughly tested to ensure lot-to-lot reproducibility.
- Potency validated in blasticidin-sensitive and blasticidin-resistant mammalian cell lines
- Absence of long-term effects confirmed in blasticidin-resistant cells
References:
1. Izumi M. et al., 1991. Blasticidin S-resistance gene (bsr): A novel selectable marker for mammalian cells. Exp.Cell Res.197:229-33.
2. Perez-Gonalez J. et al., 1990. Cloning and characterization of the gene encoding a blasticidin S acetyltransferase from Streptoverticillum sp. Gene. 86:129-34.
3. Kimura M. et al., 1994. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. Biochim. Biophys. Acta. 1219:653-9.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
10 mg/ml
Eukaryotic selection/stable cell line generation (tested), bacterial selection (tested)
C17H26N8O5 • HCl
HEPES
7.1 - 7.5
1 - 10 μg/ml (mammalian cells), 25 - 100 μg/ml (E. coli)
0.2 µm filtration
< 1 EU/mg
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Blasticidin (10 mg/ml solution)
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Cat code:ant-bl-05
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Quantity:50 mg (5 x 1 ml)
Shipping & Storage
- Shipping method: Room temperature
- 4°C or -20°C
- Avoid repeated freeze-thaw cycles
- This product is a harmful compound. Refer to SDS for handling instructions
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
Need a CoA ?
FAQ
Selective antibiotics general questions
Indeed, any selective antibiotics can be used together at the same time and in any combination.
We recommend not adding selective antibiotics until the cells have been passaged twice as cells are fragile during the initial culture procedure and the antibiotics may reduce cell viability. The resistance marker has to be expressed enough for the cells to acquire the resistance to these selective antibiotics.
For deactivation of all our selective antibiotics (Zeocin®, Hygromycin, G418, Phleomycin, Puromycin, and Blasticidin), we would recommend adding to the growth medium:
- - 5% NaOH (Sodium hydroxide)
- - 0.1% NaClO (Sodium hypochlorite)
You should then leave to react for 5 – 10 minutes and this should effectively deactivate all antibiotics in media.
Unfortunately we do not have data on the half-life of our selective antibiotics in cell culture medium. In house we prefer to add the selective antibiotics extemporaneously however if necessary you can supplement the medium with the antibiotic of your choice and keep at 4°C for no more than 1 week.
All of our selection antibiotics are shipped at room temperature. Upon receipt, they should be stored at 4°C or -20°C. Avoid repeated freeze-thaw cycles.
The expiry date is specified on the product label.
Once opened they remain stable for 1 year at -20°C providing you avoid repeated freeze-thaw cycles and they can be kept at 4°C for 3 months providing they are kept in sterile conditions.
