HEK-Blue™ Selection

Use of selective antibiotics with HEK-Blue™ cells

Antibiotics for maintenance of HEK-Blue™ Cells

HEK-Blue™ Selection is a solution that combines several selective antibiotics.

These antibiotics guarantee the persistent expression of the various transgenes introduced in HEK-Blue™ TLR2 cells, HEK-Blue™ TLR4 cells, and numerous other HEK-Blue™ cells as listed below.

Key features of HEK-Blue™ Selection

➔ Convenient

1 single tube that combines several selective antibiotics.

➔ Simple

Add directly to the culture at a 1:250 dilution.

➔ Optimized concentration

The concentration of antibiotics has been optimized to maximize their effectiveness for the maintenance of these cells.

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Specifications

Quality Control: Each lot of HEK-Blue™ Selection is thoroughly tested to ensure the absence of lot-to-lot variation.

HEK-Blue™ Selection should be used with the following cell lines:

• HEK-Blue™-2 cells
• HEK-Blue™-4 cells
• HEK-Blue™ G-CSF cells
• HEK-Blue™ hTLR2 cells
• HEK‑Blue™ hTLR2-TLR1 cells
• HEK‑Blue™ hTLR2-TLR6 cells
• HEK‑Blue™ hTLR2 KO-TLR1/6 cells
• HEK-Blue™ mTLR2 cells
• HEK-Blue™ hTLR4 cells
• HEK-Blue™ mTLR4 cells
• HEK-Blue™ IL-1R cells
• HEK-Blue™ IL-5 cells
• HEK-Blue™ IL-6 cells
• HEK-Blue™ IL-7 cells
• HEK-Blue™ IL-9 cells
• HEK-Blue™ IL-10 cells
• HEK-Blue™ IL-11 cells
• HEK-Blue™ IL-12 cells
• HEK-Blue™ IL-17 cells
• HEK-Blue™ IL-17C cells
• HEK-Blue™ IL-18 cells
• HEK-Blue™ IL-23 cells
• HEK-Blue™ IL-31 cells
• HEK-Blue™ IL-33 cells
• HEK-Blue™ CD122/CD132 cells
• HEK-Blue™ GM-CSF cells
• HEK-Blue™ STAT6-hSTING-R232 cells
• HEK-Blue™ TPO cells
• HEK-Blue™ TSLP cells

Contents

HEK-Blue™ Selection is provided as 10 x 1 ml tubes of 250X solution.

HEK-Blue™ Selection is shipped at room temperature.

Upon receipt, it should be stored at 4 °C or -20 °C.

Avoid repeated freeze-thaw cycles.