Puromycin

Selection antibiotic for bacteria and mammalian cells – Sterile, endotoxin-tested

Puromycin is a selection antibiotic for mammalian, insect, and bacterial cells, available as a sterile, cell culture-tested solution. Puromycin dihydrochloride is an aminonucleoside antibiotic naturally produced by Streptomyces alboniger as a tyrosyl tRNA analog.

Puromycin specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic is highly active on Gram-positive bacteria, mammalian and insect cells but is weakly active on Gram-negative bacteria.

Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase isolated from a Streptomyces strain [1]. Vectors carrying only one drug resistance marker for selection can be designed with the Pac gene. 

The recommended working concentrations are 0.5 to 10 μg/ml for selection in mammalian cells and 100 to 125 μg/ml for selection in E. coli.

Key features

• Produced in InvivoGen's facilities
• Each lot is thoroughly tested to ensure lot-to-lot reproducibility.
• Potency validated in puromycin-sensitive and puromycin-resistant mammalian cell lines
• Absence of long-term effects confirmed in puromycin-resistant cells
   

Reference:

1. Lacalle R. et al., 1989. Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger. Gene. 79:375-80.