Mouse TLR2 Reporter HEK 293 Cells

NF-κB-SEAP reporter cells

ABOUT

NF-κB–SEAP reporter HEK293 cells expressing mouse TLR2

HEK-Blue™-mTLR2 cells were obtained by co-transfection of the murine TLR2 and SEAP (secreted embryonic alkaline phosphatase) reporter genes into HEK293 cells.

The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.

Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response. Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induces the production of SEAP.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ mTLR2 cells is HEK-Blue™ Null2 cells.

 

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Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Species
Human
Target

TLR2

Target species

Mouse

Tested applications

TLR2 activation cellular assays

Cell type
Epithelial
Growth properties
Adherent
Tissue origin
Human embryonic kidney cells
Reporter gene
SEAP
Detection method
Colormetric
Antibiotic resistance
Blasticidin
Hygromycin
Zeocin®
Growth medium

Complete DMEM (see TDS)

Mycoplasma-free

Verified using Plasmotest™

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    HEK-Blue™ mTLR2 Cells
  • Cat code: 
    hkb-mtlr2
  • Quantity: 
    3-7 x 10^6 cells
Includes:
  • 2 x 1 ml 250X HEK-Blue™ Selection
  • 1 ml Normocin™ (50 mg/ml)
  • 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid nitrogen vapor
    Stability: 20 passages

DOCUMENTS

Documents

HEK-Blue™ mTLR2 Cells

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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