Mouse TLR2 Antibody

Mouse IgG2a (clone C9A12) - Hybridoma

ABOUT

Murine TLR2 Detection and Neutralizing Antibody (clone C9A12) - Monoclonal Mouse IgG2a

Anti-mTLR2-IgG2a (clone C9A12) is a fully mouse monoclonal antibody specific for murine Toll-like receptor 2 (TLR2, CD282). This autoantibody was raised in mice by a proprietary method designed to induce the production of anti-TLR2 antibodies directly in the animal.
Anti-mTLR2-IgG has been selected for its ability to efficiently neutralize the biological activity of mTLR2. It can also be used to detect mTLR2 using flow cytometry. This antibody has been produced in hybridomas and purified by affinity chromatography.  

TLR2 plays an essential role in detecting a diverse range of microbial pathogen-associated molecular patterns (PAMPs) [1]. An essential feature of TLR2 is its ability to form heterodimers with TLR1 and TLR6. TLR2 cooperates with TLR6 in response to diacylated mycoplasmal lipoproteins [2], and associates with TLR1 to recognize triacylated lipoproteins [3,4].
 

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Key features:

  • Reacts with murine TLR2
  • Provided azide-free
  • Each lot is functionally tested

 

Read our review Read our review on TLR2

 

References

1. Oliveira-Nascimento L. et al., 2012. The Role of TLR2 in Infection and Immunity. Front Immunol 3:79.
2. Girard R. et al., 2003. Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2. J Cell Sci. 116:293-302.
3. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. PNAS USA. 97:13766-71.
4. Thakran S. et al., 2008. Identification of Francisella tularensis lipoproteins that stimulate the Toll-like receptor (TLR) 2/TLR1 heterodimer. J Biol Chem 283:3751-9.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

Murine TLR2 (mTLR2)

Applications

Neutralization/block, Flow cytometry

Isotype
mIgG2a
Clone
C9A12
Source
Hybridoma
Purification
Protein G
Preservative
Azide-free
Reconstitution buffer
Endotoxin-free water (not provided).
Tested applications

Neutralization using cellular assays, Flow cytometry

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-mTLR2-IgG
  • Cat code: 
    mabg-mtlr2-2
  • Quantity: 
    2 x 100 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • Upon receipt, store at -20°C.
    Stability: Reconstituted antibody is stable for 1 month at 4 °C and for 1 year at -20 °C.

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Toll-Like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionarily conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, TLR1 to TLR9, TLR11, TLR12 (aka TLR11), and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR2 is involved in the recognition of a wide array of microbial molecules. TLR2 recognizes lipoteichoic acid and lipoprotein from gram-positive bacteria, lipoarabinomannan from mycobacteria, and zymosan from yeast cell wall. Moreover, TLR2 participates in the recognition of some types of LPS. TLR2 is known to heterodimerize with other TLRs, a property believed to extend the range of microbial molecules that TLR2 can recognize. TLR2 cooperates with TLR6 in response to diacylated mycoplasmal lipopeptides [1], and associates with TLR1 to recognize triacylated lipopeptides [2]. Furthermore, pathogen recognition by TLR2 is strongly enhanced by CD14 [3].

 

1. Girard R et al., 2003. Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2. J Cell Sci. 116(Pt 2):293-302.
2. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. Proc Natl Acad Sci USA. 97(25):13766-71.
3. Lotz S. et al., 2004. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 75(3):467-77.

DOCUMENTS

Documents

Anti-mTLR2-IgG

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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Citations

CUSTOMER SERVICE & TECHNICAL SUPPORT

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