Anti-mTLR2 Detection and Neutralizing mAb

Murine TLR2 Detection and Neutralizing Antibody (clone C9A12) - Monoclonal Mouse IgG2a

Neutralizing and detection antibody against mTLR2

Anti-mTLR2-IgG2a (clone C9A12) is a fully mouse monoclonal antibody specific for murine Toll-like receptor 2 (TLR2, CD282). This autoantibody was raised in mice by a proprietary method designed to induce the production of anti-TLR2 antibodies directly in the animal. Anti-mTLR2-IgG has been selected for its ability to efficiently neutralize the biological activity of mTLR2. It can also be used to detect mTLR2 using flow cytometry. This antibody has been produced in hybridomas and purified by affinity chromatography.  
TLR2 plays an essential role in detecting a diverse range of microbial pathogen-associated molecular patterns (PAMPs) [1]. An essential feature of TLR2 is its ability to form heterodimers with TLR1 and TLR6. TLR2 cooperates with TLR6 in response to diacylated mycoplasmal lipoproteins [2], and associates with TLR1 to recognize triacylated lipoproteins [3,4].

More details

Key features:

• Reacts with murine TLR2
• Provided azide-free
• Each lot is functionally tested

Read our review on TLR2

References

1. Oliveira-Nascimento L. et al., 2012. The Role of TLR2 in Infection and Immunity. Front Immunol 3:79.
2. Girard R. et al., 2003. Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2. J Cell Sci. 116:293-302.
3. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. PNAS USA. 97:13766-71.
4. Thakran S. et al., 2008. Identification of Francisella tularensis lipoproteins that stimulate the Toll-like receptor (TLR) 2/TLR1 heterodimer. J Biol Chem 283:3751-9.

Specifications

Target: Murine TLR2 (mTLR2)

Specificity: No cross-reactivity with human TLR2

Clonality: Monoclonal antibody

Clone: C9A12

Isotype: Mouse IgG2a

Control: Mouse Control IgG2a 

Source: Hybridoma cells

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine, and stabilizing agents

Purity: Purified by affinity chromatography with protein G

Application: Neutralization/block; Flow cytometry

Quality control:

• This product has been validated for neutralization using cellular assays.
• The binding of Anti-mTLR2-IgG to mTLR2 on cells has been validated using flow cytometry.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 2 x 100 µg of purified monoclonal anti-mTLR2 IgG antibody, provided lyophilized.

Product is shipped at room temperature.

Upon receipt, store at -20°C.

Details

Toll-Like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionarily conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, TLR1 to TLR9, TLR11, TLR12 (aka TLR11), and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR2 is involved in the recognition of a wide array of microbial molecules. TLR2 recognizes lipoteichoic acid and lipoprotein from gram-positive bacteria, lipoarabinomannan from mycobacteria, and zymosan from yeast cell wall. Moreover, TLR2 participates in the recognition of some types of LPS. TLR2 is known to heterodimerize with other TLRs, a property believed to extend the range of microbial molecules that TLR2 can recognize. TLR2 cooperates with TLR6 in response to diacylated mycoplasmal lipopeptides [1], and associates with TLR1 to recognize triacylated lipopeptides [2]. Furthermore, pathogen recognition by TLR2 is strongly enhanced by CD14 [3].

1. Girard R et al., 2003. Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2. J Cell Sci. 116(Pt 2):293-302.
2. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. Proc Natl Acad Sci USA. 97(25):13766-71.
3. Lotz S. et al., 2004. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 75(3):467-77.