HEK-Blue™ Null2 Cells

NF-κB-SEAP parental cell line

HEK-Blue™ Null2 cells express the SEAP reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1 binding sites.

HEK-Blue™ Null2 is the parental cell line of several HEK-Blue™ TLR and HEK-Blue™ NOD cells:

• HEK-Blue™ mTLR2
• HEK-Blue™ hTLR4
• HEK-Blue™ hMD2 CD14
• HEK-Blue™ hNOD2 and HEK-Blue™ mNOD2

Specifications

Antibiotic resistance: Zeocin®

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

This product is covered by a Limited Use License (See Terms and Conditions).

Contents

• 1 vial containing 3-7 x 10⁶ cells
• 100 μl Zeocin® (100 mg/ml)
• 1 ml Normocin™ (50 mg/ml)
• 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)

Shipped on dry ice (Europe, USA, Canada and some areas in Asia)