HEK-Blue™ Null1-v Cells

Parental NF-κB inducible SEAP cell line

HEK-Blue™ Null1-v cells are derived from human embryonic kidney 293 (HEK293) cells. HEK-Blue™ Null1-v cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1 binding sites. Stimulation of HEK-Blue™ Null1-v cells with NF-κB and/or AP-1 activators induces the production of SEAP. These cells express endogenous levels of TLR3, TLR5, NOD1, ALPK1, and TIFA.

The HEK-Blue™ Null1-v cell line is the parental cell line of:

• HEK-Blue™ mTLR4 cells 
• HEK‑Blue™ mTLR5 cells
• HEK-Blue™ hTLR7 cells
• HEK-Blue™ mTLR8 cells
• HEK-Blue™ KO-ALPK1 cells
• HEK-Blue™ KO-TIFA cells

Levels of SEAP produced by HEK-Blue™ Null1-v cells can be easily determined using HEK‑Blue™ Detection: A cell culture medium that contains all the required nutrients for cell growth, as well as a specific SEAP color substrate that allows for real-time detection of SEAP activity. The hydrolysis of the substrate by SEAP produces a purple/blue color that can be easily detected with the naked eye or quantitatively measured with a spectrophotometer (OD 620-655 nm). HEK-Blue™ Detection can be used in high-throughput screening (HTS).

Additionally, QUANTI-Blue™ Solution, a SEAP detection and quantification reagent can also be used. It allows the same cell culture to be repeatedly sampled for kinetic studies or further experimentation.

Specifications

Growth medium:DMEM, 4.5 g/L glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin, 100 µg/ml streptomycin, 100 µg/ml 

Antibiotic resistance: Zeocin®

Quality Control:

• Activation of the NF-κB response has been verified upon stimulation with various inducers (e.g. TNF-α and IL-1β).
• The stability for 20 passages, following thawing, has been verified. 
• These cells are guaranteed mycoplasma-free. 

Contents

• 3-7 x 10⁶ HEK-Blue™ Null1-v cells in a cryovial or shipping flask
• 1 ml of Zeocin® (100 mg/ml)
• 1 ml of Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria, and fungi.
• 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)

Shipped on dry ice (Europe, USA, Canada and some areas in Asia)