ISG Dual Reporter 293 Cells - 293-Dual™ Null Cells

293-Dual™ Null Cells (ISG-SEAP/KI-[IFN-β]Lucia)

293-Dual™ Null (ISG/KI-IFNb) cells are the parental cell line for the collection of 293-Dual™ STING (ISG/KI-IFNb) reporter cells. These 293-derived cells allow a dual monitoring of the ISG/IFN pathway as they express two different reporter constructs, featuring the secreted embryonic alkaline phosphatase (SEAP) and Lucia (encoding a secreted luciferase) reporter genes. 293-Dual™ Null cells stably express an ISRE-inducible SEAP reporter construct, as well as the Lucia gene placed under the control of the endogenous IFN-β promoter; the coding sequence of IFN-β has been replaced by the Lucia luciferase ORF using knockin technology.

293-Dual™ Null cells do not respond to cyclic dinucleotides (CDNs), similarly to other 293-derived cells which are known to have a non-functional STING pathway [1,2].

They are resistant to hygromycin and Zeocin®.

References:

1. Burdette DL. & Vance RE., 2013. STING and the innate immune response to nucleic acids in the cytosol. Nat Immunol. 14(1):19-26.
2. Diner E. et al., 2013. The innate immune DNA sensor cGAS produces a noncanonical cyclic dinucleotide that activates human STING. Cell Rep. 3(5):1355-61.