HEK-Blue™ hTLR5 Cells
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Cat.code:
hkb-htlr5
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ABOUT
NF-κB–SEAP reporter HEK293 cells expressing human TLR5
HEK-Blue™-hTLR5 cells were obtained by co-transfection of the human TLR5 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR5 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR5 cells is HEK-Blue™ Null1 cells.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
TLR5 activation cellular assays
Complete DMEM (See TDS)
Verified using Plasmotest™
Each lot functionally tested and validated
CONTENTS
Contents
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Product:HEK-Blue™ hTLR5 Cells
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Cat code:hkb-htlr5
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Quantity:3-7 x 10^6 cells
1 ml of Blasticidin (10 mg/ml) 1 ml of Zeocin™ (100 mg/ml) 1 ml of Normocin™ (50 mg/ml) 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipping & Storage
- Shipping method: Dry ice
- Liquid Nitrogen Vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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