Human TLR5 Reporter HEK 293 Cells

NF-κB-SEAP reporter cells

ABOUT

NF-κB–SEAP reporter HEK293 cells expressing human TLR5

HEK-Blue™-hTLR5 cells were obtained by co-transfection of the human TLR5 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.

The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR5 ligand activates NF-κB and AP-1 which induce the production of SEAP.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ hTLR5 cells is HEK-Blue™ Null1 cells.

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

SPECIFICATIONS

Specifications

Species
Human
Tested applications

TLR5 activation cellular assays

Cell type
Epithelial
Growth properties
Adherent
Tissue origin
Human embryonic kidney cells
Reporter gene
SEAP
Antibiotic resistance
Blasticidin
Zeocin®
Growth medium

Complete DMEM (See TDS)

Mycoplasma-free

Verified using Plasmotest™

Quality control

Each lot functionally tested and validated

CONTENTS

Contents

  • Product: 
    HEK-Blue™ hTLR5 Cells
  • Cat code: 
    hkb-htlr5
  • Quantity: 
    3-7 x 10^6 cells
Notes:

1 ml of Blasticidin (10 mg/ml) 1 ml of Zeocin™ (100 mg/ml) 1 ml of Normocin™ (50 mg/ml) 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)

Shipping & Storage

  • Shipping method:  Dry ice
  • Storage:

    • Liquid Nitrogen Vapor
    Stability: 20 passages

    Caution:

    • Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.

DOCUMENTS

Documents

HEK-Blue™ hTLR5 Cells

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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