Human TLR5 Reporter HEK293 Cells (NF-κB)
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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HEK-Blue™ hTLR5 Cells Human TLR5 expressing HEK293 reporter cells (NF-κB pathway) |
Show product |
3-7 x 10e6 cells |
hkb-htlr5
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NF-κB–SEAP reporter HEK293 cells expressing human TLR5
HEK-Blue™-hTLR5 cells were obtained by co-transfection of the human TLR5 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR5 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR5 cells is HEK-Blue™ Null1 cells.
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Specifications
Antibiotic resistance: blasticidin, Zeocin®.
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.
Guaranteed mycoplasma-free
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- 1 vial containing 3-7 x 106 cells
- 1 ml of Blasticidin (10 mg/ml)
- 1 ml of Zeocin™ (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipped on dry ice (Europe, USA, Canada, and some areas in Asia)
FAQ Cell Lines
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Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.