pMOD2-Blast
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Cat.code:
pmod2-blast
- Documents
Synthetic blasticidin resistance gene in cloning plasmid
Resistance to the antibiotic Blasticidin S is conferred by the bsr gene from Bacillus cereus.
The DNA sequence of this gene was modified by optimizing the codon usage and reducing the number of CpG motifs from 14 to 4 without changing the amino acid sequence of the wild type protein.
The low-CpG Bsr gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin.
To facilitate the excision and subcloning of the low-CpG Bsr gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.
Specifications
Gene: Low-CpG Bsr
Description: Synthetic Blasticidin resistance gene
Backbone: pMOD2
Selection: Ampicillin
14 CpG in the native gene, 4 CpG in the synthetic gene
Contents
- 20 µg of lyophilized DNA
Product is shipped at room temperature.
Lyophilized DNA should be resuspended upon receipt and stored at -20°C.
DOCUMENTS
Documents
Technical Data Sheet
Plasmid Sequence
Safety Data Sheet
Certificate of analysis
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