CpG-free neo

Synthetic neomycin resistance gene in cloning plasmid

CpG-free neo confers resistance to Kanamycin in E. coli and G418 in mammalian cells.

This DNA sequence is based on the Tn5 aph2 gene and encodes aminoglycoside 3‘-phosphotransferase, APH 3‘ II.

The gene was modified by optimizing the codon usage and deleting the CpG motifs without changing the amino acid sequence of the wild type protein.

The synthetic neo gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin.

To facilitate the excision and subcloning of the synthetic neo gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.