CpG-free GFP::Sh

Synthetic reporter / resistance fusion gene in expression plasmid

ABOUT

Synthetic reporter / resistance fusion gene in expression plasmid

GFP::Sh is a fusion gene that encodes a red-shifted variant of the jellyfish green fluorescent protein (GFP) and the Sh ble gene conferring resistance to Zeocin™.

Expression of GFP::Sh can be detected by fluorescent microscopy or flow cytometry. This fusion protein absorbs blue light (major peak at 480 nm) and emits green light (major peak at 505 nm).

The CpG-free GFP::Sh fusion gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in both mammalian and E.coli cells. To facilitate subcloning, the GFP::Sh gene is flanked by two unique restriction sites.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

In vitro transfection, fluorescent microscopy, flow cytometry

Gene
CpG-free GFP::Sh
Plasmid backbone
pSELECT-zeo
Antibiotic resistance
Zeocin®
Gene promoter
Human EF1α/HTLV hybrid promoter
Reporter gene
GFP
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Reconstitution buffer
Sterile water (not provided)
Tested applications

in vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

Additional information

110 CpG in the native gene

CONTENTS

Contents

  • Product: 
    pSELECT-zeo-GFPSh
  • Cat code: 
    psetz-zgfpsh
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml) 

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20 °C
    Stability: -20 °C for up to 3 months

DOCUMENTS

Documents

pSELECT-zeo-GFPSh

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

Need a CoA ?

CUSTOMER SERVICE & TECHNICAL SUPPORT

Question about this product ?