CpG-free LacZ

Synthetic LacZ reporter gene in expression plasmid

The synthetic LacZ gene is a humanized and CpG-free allele of the LacZ gene.

The lacZ gene codes for the enzyme β-galactosidase which catalyzes the hydrolysis of the substrate X-Gal to produce a blue color that is easily visualized under a microscope.

This CpG-free gene is ten times more active than the wild-type gene in mammalian cells.

The CpG-free LacZ gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in both mammalian and E.coli cells.

References:

1. Kim, D.W. et al. (1990).Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system.Gene 2: 217-223.
2. Takebe, Y. et al. (1988).SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat.Mol. Cell Biol. 1: 466-472.
3. Carswell, S., & Alwine, J.C. (1989).Efficiency of utilization of the simian virus 40 late polyadenylation site: effects of upstream sequences.Mol. Cell Biol. 10: 4248-4258.
4. Yu J & Russell JE. (2001).Structural and functional analysis of an mRNP complex that mediates the high stability of human beta-globin mRNA.Mol Cell Biol, 21(17):5879-88.