Low-CpG pac

Synthetic puromycin resistance gene in cloning plasmid

Resistance to the antibiotic puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase isolated from a Streptomyces strain.

The DNA sequence of this gene was modified by optimizing the codon usage and reducing CpG motifs to 16 without changing the amino acid sequence of the wild type protein.

The low-CpG Pac gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin.

To facilitate the excision and subcloning of the low-CpG Pac gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.