TLR 9
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
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pUNO1-bTLR9 pUNO1 bearing the bovine TLR9 gene |
Show product |
20 µg |
puno1-btlr9
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pUNO1-hTLR9a pUNO1 bearing the human TLR9 gene, isoform_1 |
Show product |
20 µg |
puno1-htlr9a
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pUNO3-hTLR9a pUNO3 bearing the human TLR9 gene, isoform_1 |
Show product |
20 µg |
puno3-htlr9a
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pUNO1-mTLR9 pUNO1 bearing the mouse TLR9 gene |
Show product |
20 µg |
puno1-mtlr9
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pUNO1-pTLR9 pUNO1 bearing the pig TLR9 gene |
Show product |
20 µg |
puno1-ptlr9
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Expression-ready ORF Clones
Alias : TLR9, CD289
Toll Like Receptor 9
TLR9 is expressed in the endoplasmic reticulum of resting cells in contrast to most TLRs that are located on the plasma membrane [1]. TLR9 recognizes specific unmethylated CpG-ODN sequences, that distinguish bacterial DNA from mammalian DNA. TLR9 engages an intracellular signaling pathway that involves MyD88 leading to NF-κB translocation [2]. TLR9 recognizes different CpG motifs; the optimal sequences being GTCGTT and GACGTT for human TLR9 and murine TLR9 respectively [3]. TLR9 binds directly to CpG DNA and recognizes specifically CpG DNA that is unmethylated and single stranded [4].
1. Latz E. et al., 2004. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol. 5(2):190-8.
2. Chuang TH. et al., 2002. Toll-like receptor 9 mediates CpG-DNA signaling. J Leukoc Biol, 71(3):538-44.
3. Bauer S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc Natl Acad Sci USA, 98(16):9237-42.
4. Rutz M. et al., 2004. Toll-like receptor 9 binds single-stranded CpGDNA in a sequence- and pH-dependent manner. Eur J Immunol. 34(9):2541-50.
Specifications
Bovine TLR9 (pUNO1-bTLR9)
Genbank : EF076731
ORF size : 3090 bp
Subclone : AgeI - NheI
Human TLR9 isoform 1 (pUNO1-hTLR9a)
ORF size : 3099 bp
Subclone : MluI - NheI
Human TLR9 isoform 1 (pUNO3-hTLR9a)
ORF size : 3099 bp
Mouse TLR9 (pUNO1-mTLR9)
ORF size : 3099 bp
Subclone : AgeI - NheI
Pig TLR9 (pUNO1-pTLR9)
Genbank : AY859728.1
ORF size : 3093 bp
Subclone : AgeI - NheI
pUNO-TLR Contents
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
pUNO1 plamids:
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.
pUNO3 plasmid:
- 20 µg of lyophilized DNA
- 1 ml Hygromycin B Gold (hygromycin) at 100 mg/ml
Hygromycin B is a hazardous compound. Avoid contact with skin and eyes.
Store Hygromycin B Gold at 4°C or -20˚C.
Avoid repeated freeze-thaw cycles.