pDUO-CD14/TLR4

pDUO plasmid co-expressing CD14 and TLR4 genes

pDUO-CD14/TLR4 is an expression vector designed to co-express CD14 and TLR4 genes known to interact with each other : human CD14 (1125 bp) and human TLR4 (2517 bp) or murine CD14 (480 bp) and murine TLR4 (2505 bp).

The genes cloned into pDUO comprise the coding sequence (without introns) from the ATG to the Stop codon.

Specifications

pDUO plasmid with human CD14 and human TLR4 isoform A genes.
Name: CD14 / TLR4A
Species: Human
Backbone: pDUO
Selection: Blasticidin

pDUO2 plasmid with murine CD14 and murine TLR4 genes.
Name: CD14 / TLR4
Species: Mouse
Backbone: pDUO2
Selection: Hygromycin

Contents

pDUO-hCD14/TLR4A:

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

pDUO2-mCD14/TLR4:

• 20 µg of lyophilized DNA
• 1 ml of Hygromycin B Gold (hygromycin) at 100 mg/ml

Description

TLR4 is the receptor for Gram-negative lipopolysaccharide (LPS). The TLR4 gene was shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to LPS [1]. However, TLR4 alone is not sufficient to confer LPS responsiveness.
TLR4 requires MD-2, a secreted molecule, to functionaly interact with LPS [2]. TLR4 physically associates with MD2, and together with a third protein called CD14, this complex is responsible for LPS recognition and signaling [3].

1. Poltorak A. et al., 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science, 282(5396):2085-8.
2. Nagai Y. et al., 2002. Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat Immunol. 3(7):667-72
3. da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24):21129-35

Details