TLR 5
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
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pUNO1-bTLR5 pUNO1 bearing the bovine TLR5 gene |
Show product |
20 µg |
puno1-btlr5
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pUNO1-hTLR5 pUNO1 bearing the human TLR5 gene |
Show product |
20 µg |
puno1-htlr5
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pUNO3-hTLR5 pUNO3 bearing the human TLR5 gene |
Show product |
20 µg |
puno3-htlr5
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pUNO1-mTLR5 pUNO1 bearing the mouse TLR5 gene |
Show product |
20 µg |
puno1-mtlr5
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pUNO1-pTLR5 pUNO1 bearing the pig TLR5 gene |
Show product |
20 µg |
puno1-ptlr5
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Expression-ready ORF Clones
Alias : TLR5, CD285
Toll Like Receptor 5
TLR5 is the Toll-like molecule that recognizes flagellin from both Gram-positive and Gram-negative bacteria. TLR5 was identified by the presence of the TIR domain and is expressed in spleen, peripheral blood leukocytes and epithelial cells.
Activation of the receptor stimulates the production of proinflammatory cytokines, such as TNF-α, through signaling via the adaptor protein MyD88 and the serine kinase IRAK [1, 2]. TLR5 can generate a proinflammatory signal as a homodimer suggesting that it might be the only TLR required for flagellin recognition [2].
1. Gewirtz AT. et al. (2001). J Immunol, 167(4):1882-5.
2. Hayashi F. et al. (2001). Nature, 410(6832):1099-103.
Specifications
Bovine TLR5 (pUNO1-bTLR5)
ORF size : 2577 bp
Subclone : NcoI - NheI
Human TLR5 (pUNO1-hTLR5)
Genbank : NM_003268.6
ORF size : 2577 bp
Subclone : NcoI - NheI
Human TLR5 (pUNO3-hTLR5)
ORF size : 2577 bp
Subclone : NcoI - NheI
Mouse TLR5 (pUNO1-mTLR5)
ORF size : 2580 bp
Subclone : AgeI - NheI
Pig TLR5 (pUNO1-pTLR5)
ORF size : 2571 bp
Subclone : AgeI - NheI
pUNO-TLR Contents
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
pUNO1 plamids:
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.
pUNO3 plasmid:
- 20 µg of lyophilized DNA
- 1 ml Hygromycin B Gold (hygromycin) at 100 mg/ml
Hygromycin B is a hazardous compound. Avoid contact with skin and eyes.
Store Hygromycin B Gold at 4°C or -20˚C.
Avoid repeated freeze-thaw cycles.