TLR6-DN-HA

Toll-Like Receptor 6 ΔTIR (Dominant Negative TLR6), HA-tagged

Expression-ready ORF Clones

Alias : TLR6, CD286

TLR6-DN (ΔTIR) : Dominant Negative TIR-less TLR6 gene

 

TLR6 is expressed in spleen and BPL and, acts as a co-receptor with TLR2 to recognize peptidoglycan and the yeast cell wall particle, zymosan [1,2]. Furthermore, TLR6- and TLR2-deficient mice were reported to be hyporesponsive to mycoplasma macrophage-activating lipopeptide-2 kD (MALP-2), a diacylated lipoprotein, suggesting that TLR2 and TLR6 coordinate the response to this ligand. By contrast, TLR2 is able to recognize bacterial lipoproteins triacylated at the N-terminus cysteine residue [3]. Thus TLR6 appears to discriminate between the N-terminal lipoylated structures of MALP-2 and lipopeptides derived from other bacteria.
TLR6-DN is a TIR-less form of the TLR6 gene generated by deleting the TIR domain (468 bp). This truncated TLR6 gene is still able to recognize its ligands but is unable to induce the signaling cascade. The gene has been fused at the 3'end to the influenza hemagglutinin (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.

 

References

1. Ozinsky A. et al., 2000. Proc Natl Acad Sci U S A 97(25):13766-71.
2. Underhill D.M., 2003. J Endotoxin Res. 9(3):176-80.
3. Takeuchi O. et al. 2001. Int Immunol. 13(7):933-40.

Specifications

Human TLR6-DN-HA (pUNO1-hTLR6-DN-HA3x)

ORF size : 2016 bp
Subclone : AgeI - NheI

Mouse TLR6-DN-HA (pUNO1-mTLR6-DN-HA3x)

ORF size : 2049 bp
Subclone : NcoI - NheI

Contents

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

stability Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Alert Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.

DOCUMENTS

Documents

pUNO1-hTLR6-DN-HA3x

Technical Data Sheet

Plasmid Map and Sequence

Plasmid Sequence

Certificate of analysis

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