TLR7-DN

Toll-Like Receptor 7 ΔTIR (Dominant Negative TLR7)

Expression-ready ORF Clones

Alias : TLR7, CD287

TLR7-DN (ΔTIR) : Dominant Negative TIR-less TLR7 gene

 

TLR7 is phylogenetically close to TLR8 and TLR9 and has a higher molecular weight when compared with hTLR1-6, largely as a result of a longer ectodomain [1]. TLR7 recognizes single stranded RNAs and small synthetic molecules such as imidazoquinolines and nucleoside analogs [2,3].
TLR7-DN is a TIR-less form of the TLR7 gene generated by deleting the TIR domain (480 bp). This truncated TLR7 gene is still able to recognize its ligands but is unable to induce the signaling cascade.

 

1. Chuang TH. & RJ. Ulevitch (2000). Eur Cytokine Netw, 11(3):372-8.
2. Hemmi H. et al (2002). Nat Immunol, 3(2):196-200.
3. Lee J et al. (2003). Proc Natl Acad Sci U S A, 100(11):6646-6651

Specifications

Human TLR7-DN (pUNO1-hTLR7-DN)

ORF size : 2670 bp
Subclone : AgeI - NheI

Mouse TLR7-DN (pUNO1-mTLR7-DN)

ORF size : 2673 bp
Subclone : AgeI - NheI

Contents

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

stability Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Alert Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.

DOCUMENTS

Documents

pUNO1-hTLR7-DN

Technical Data Sheet

Safety Data Sheet

Plasmid Map and Sequence

Plasmid Sequence

Certificate of analysis

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