TLR9-DN

Expression-ready ORF Clones

Alias : TLR9, CD289

TLR9-DN (ΔTIR) : Dominant Negative TIR-less TLR9 gene

TLR9, which is localized intracellularly, is involved in the recognition of specific unmethylated CpG-ODN sequences, that distinguishes bacterial DNA from mammalian DNA. TLR9 engages an intracellular pathway that involves MyD88 leading to NF-κB translocation [1]. TLR9 has been shown to recognize different CpG motifs; the optimal sequences being GTCGTT and GACGTT for hTLR9 and mTLR9 respectively [2]. The ectodomains of hTLR9 and mTLR9 present distinct distribution patterns of leucine-rich repeats. The sequence differences observed in the ectodomains of h- and m-TLR9 may account for the differences in ligand specificity in human versus murine TLR9.
TLR9-DN is a TIR-less form of the TLR9 generated by deleting the TIR domain (489 bp). This truncated TLR9 gene is still able to recognize its ligands but is unable to induce the signaling cascade.

1. Chuang TH. et al. (2002). J Leukoc Biol, 71(3):538-44.
2. Bauer S. et al. (2001). Proc Natl Acad Sci U S A, 98(16):9237-42.