pUNO1-hTLR8-DN-HA
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Cat.code:
puno1ha-htlr8-dn
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Expression-ready ORF Clones
Alias : TLR8, CD288
TLR8-DN (ΔTIR) : Dominant Negative TIR-less TLR8 gene
TLR8-DN is a TIR-less form of the TLR8 generated by deleting the TIR domain (486 bp). This truncated TLR8 gene is still able to recognize its ligands but is unable to induce the signaling cascade.
The gene has been fused at the 3'end to the influenza hemagglutinin (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
Specifications
Human TLR8-DN-HA (pUNO1-hTLR8-DN-HA)
Subclone : AgeI - NheI
Mouse TLR8-DN-HA (pUNO1-mTLR8-DN-HA)
Subclone : AgeI - NheI
Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.
DOCUMENTS
Documents
Technical Data Sheet
Certificate of analysis
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