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TLR8-DN-HA

Product Unit size Cat. code Docs. Qty. Price

pUNO1-hTLR8-DN-HA

pUNO1 bearing the human TLR8-DN gene, with HA-tag

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20 µg

puno1ha-htlr8-dn
+-
$501

pUNO1-mTLR8-DN-HA

pUNO1 bearing the mouse TLR8-DN gene, with HA-tag

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20 µg

puno1ha-mtlr8-dn
+-
$501

Expression-ready ORF Clones

Alias : TLR8, CD288

TLR8-DN (ΔTIR) : Dominant Negative TIR-less TLR8 gene

 

TLR8-DN is a TIR-less form of the TLR8 generated by deleting the TIR domain (486 bp). This truncated TLR8 gene is still able to recognize its ligands but is unable to induce the signaling cascade.

The gene has been fused at the 3'end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.

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Specifications

Human TLR8-DN-HA (pUNO1-hTLR8-DN-HA)

Subclone : AgeI - NheI

Mouse TLR8-DN-HA (pUNO1-mTLR8-DN-HA)

Subclone : AgeI - NheI

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pUNO1 Contents

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

stability Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Alert Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.

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