TIRAP

Expression-ready ORF Clones

Alias : MAL, wyatt

TIR domain containing adaptor protein.
MyD88 adapter-like.

TIRAP

TIR domain-containing adaptor protein (TIRAP), also known as Mal, is differentially involved in the signaling to NF-kB by members of the TLR family. TIRAP-deficient mice were shown to respond normally to the TLR3, TLR5, TLR7, and TLR9 ligands, but to be defective in cytokine response to LPS, a ligand for TLR4, as well as ligands for TLR2, TLR1, and TLR6 [1,2]. TIRAP contributes to TLR2 and TLR4 MyD88-dependent signaling but interacts only with TLR4 to activate a MyD88-independent pathway to induce IFN-β and STAT-1-dependent genes [3].

1. Horng T. et al. 2002. The adaptor molecule TIRAP provides signalling specificity for Toll-like receptors. Nature 420(6913):329-33
2. Yamamoto M. et al. 2002. Essential role for TIRAP in activation of the signalling cascade shared by TLR2 and TLR4. Nature 420(6913):324-9
3. Toshchakov V. et al., 2003. TLR2 and TLR4 agonists stimulate unique repertoires of host resistance genes in murine macrophages: interferon-beta-dependent signaling in TLR4-mediated responses. J Endotoxin Res. 9(3):169-75.

Specifications

Human TIRAP isoform 2 (longest) (pUNO1-hTIRAPb)

Genbank : NM_148910.2 with silent variation in codon Ala186
ORF size : 708 bp
Subclone : NcoI - NheI

Mouse TIRAP (pUNO1-mTIRAP)

Genbank : NM_001177845.1 with silent variation in codon Pro70
ORF size : 726 bp
Subclone : NcoI - NheI

pUNO1 Contents

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

Product is shipped at room temperature.

Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.