TLR7-DN-HA
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pUNO1-hTLR7-DN-HA pUNO1 bearing the human TLR7-DN gene, with HA-tag |
Show product |
20 µg |
puno1ha-htlr7-dn
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pUNO1-mTLR7-DN-HA3x pUNO1 bearing the mouse TLR7-DN gene, with HA-tag |
Show product |
20 µg |
puno1ha-mtlr7-dn
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Expression-ready ORF Clones
Alias : TLR7, CD287
TLR7-DN (ΔTIR) : Dominant Negative TIR-less TLR7 gene
TLR7 is phylogenetically close to TLR8 and TLR9 and has a higher molecular weight when compared with hTLR1-6, largely as a result of a longer ectodomain [1]. TLR7 recognizes single stranded RNAs and small synthetic molecules such as imidazoquinolines and nucleoside analogs [2,3].
TLR7-DN is a TIR-less form of the TLR7 gene generated by deleting the TIR domain (480 bp). This truncated TLR7 gene is still able to recognize its ligands but is unable to induce the signaling cascade. The gene has been fused at the 3'end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
1. Chuang TH. & RJ. Ulevitch (2000). Eur Cytokine Netw, 11(3):372-8.
2. Hemmi H. et al (2002). Nat Immunol, 3(2):196-200.
3. Lee J et al. (2003). Proc Natl Acad Sci U S A, 100(11):6646-6651
Specifications
Human TLR7-DN-HA (pUNO1-hTLR7-DN-HA)
Subclone : AgeI - NheI
Mouse TLR7-DN-HA (pUNO1-mTLR7-DN-HA3x)
ORF size : 2766 bp
Subclone : AgeI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.