TLR1-DN-HA

Expression-ready ORF Clones

Alias : TLR1, CD281

HA-Tagged TLR1-DN (ΔTIR) : Dominant Negative TIR-less TLR1 gene

TLR1, the first member of the toll-like receptor family, was identified by the presence of a domain homology found in both Drosophila Toll and human interleukin-1 receptors.
TLR1 is expressed at higher levels in the spleen and peripheral blood cells [1]. No direct ligands have been identified so far for TLR1, and its function remains unclear. TLR1 seems to act as a coreceptor. TLR1 was shown to associate with TLR2 in response to triacylated lipopeptides [2], but not diacylated lipopeptides [3]. These observations suggest that TLR1 is able to discriminate among lipoproteins by recognizing the lipid configuration. TLR1-DN is a TIR-less form of the TLR1 gene generated by deleting the TIR domain (453 bp). The gene has been fused at the 3'end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.

1. Zarember KA. & PJ. Godowski (2002). J Immunol, 168(2):554-61.
2. Takeuchi O. et al. (2002). J Immunol, 169(1):10-4.
3. Takeuchi O.et al. (2001). Int Immunol, 13(7):933-40.

Specifications

Human TLR1-DN-HA (pUNO1-hTLR1-DN-HA3x)

ORF size : 2001 bp
Subclone : AgeI - NheI

Mouse TLR1-DN-HA (pUNO1-mTLR1-DN-HA)

Subclone : AgeI - NheI

pUNO1 Contents

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

Product is shipped at room temperature.

Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.