TLR9-DN-HA

Expression-ready ORF Clones

Alias : TLR9, CD289

TLR9-DN (ΔTIR) : Dominant Negative TIR-less TLR9 gene

TLR9, which is localized intracellularly, is involved in the recognition of specific unmethylated CpG-ODN sequences, that distinguishes bacterial DNA from mammalian DNA. TLR9 engages an intracellular pathway that involves MyD88 leading to NF-κB translocation [1]. TLR9 has been shown to recognize different CpG motifs; the optimal sequences being GTCGTT and GACGTT for hTLR9 and mTLR9 respectively [2]. The ectodomains of hTLR9 and mTLR9 present distinct distribution patterns of leucine-rich repeats. The sequence differences observed in the ectodomains of h- and m-TLR9 may account for the differences in ligand specificity in human versus murine TLR9.
TLR9-DN is a TIR-less form of the TLR9 generated by deleting the TIR domain (489 bp). This truncated TLR9 gene is still able to recognize its ligands but is unable to induce the signaling cascade. The gene has been fused at the 3'end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.

1. Chuang TH. et al. (2002). J Leukoc Biol, 71(3):538-44.
2. Bauer S. et al. (2001). Proc Natl Acad Sci U S A, 98(16):9237-42.

Specifications

Human TLR9-DN-HA (pUNO1-hTLR9a-DN-HA)

Subclone : MluI - NheI

Mouse TLR9-DN-HA (pUNO1-mTLR9-DN-HA)

ORF size : 2643 bp
Subclone : AgeI - NheI

pUNO1 Contents

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

Product is shipped at room temperature.

Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.