TICAM1
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pUNO2-hTICAM1 pUNO2 bearing the human TICAM1 (TRIF) gene |
Show product |
20 µg |
puno2-hticam1
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pUNO1-hTICAM1-DN pUNO1 bearing a dominant negative version of the human TICAM1 (TRIF) gene |
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20 µg |
puno1-hticam1-dn
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pUNO1-hTICAM1-sa pUNO1 bearing the human saTICAM1 (super-activated TICAM1) gene |
Show product |
20 µg |
puno1-hticam1-sa
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pUNO1-mTICAM1 pUNO1 bearing the mouse TICAM1 (TRIF) gene |
Show product |
20 µg |
puno1-mticam1
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Expression-ready ORF Clones
Alias : TRIF
Toll like receptor adaptor molecule 1.
TIR domain-containing adapter inducing IFNbeta.
TIR-containing adaptor molecule 1.
Available versions:
- Wild type TICAM1
- Dominant negative TICAM1
TICAM1 (TRIF) is an adapter involved in the MyD88-independent signaling pathway of several TLRs. A dominant-negative form of TICAM1 was shown to completely abolish TLR3-induced signaling and also to block the NF-kB activity mediated by TLR2, TLR4 and TLR7 [1]. Further analysis has revealed that TICAM1 binds preferentially to TLR3 and TLR4 and is essential in their signaling pathways [2]. It predominantly activates IRF-3 and the production of IFN-b rather than NF-kB [3].
TICAM1-DN
Dominant negative TICAM1 (aa387-566) harbors only the TIR domain and lacks the N-terminal and C-terminal portions [1]. Furthermore, it contains a proline to histidine mutation at amino acid 434. This proline residue is conserved among TLRs and is essential for activation of the TLR-mediated signaling.
1. Yamamoto M. et al. 2002. Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-b promoter in the Toll-like receptor signaling. J Immunol, 169(12):6668-6672
2. Yamamoto M. et al., 2003. Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway. Science. 301(5633):640-3.
3. Oshiumi H. et al., 2003. TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction.Nat Immunol. 4(2):161-7.
Specifications
Human TICAM1 (pUNO2-hTICAM1)
ORF size : 2139 bp
Subclone : BspHI - NheI
Human TICAM1-DN (pUNO1-hTICAM1-DN)
ORF size : 546 bp
Subclone : NcoI - NheI
Human super-activated TICAM1 (pUNO1-hTICAM1-sa)
ORF size : 1935 bp
Subclone : BspHI - NheI
Mouse TICAM1 (pUNO1-mTICAM1)
Genbank : NM_174989.4
ORF size : 2199 bp
Subclone : AgeI - EcoRI
Contents
pUNO1-mcs:
- 20 µg of lyophilized DNA
- 2 x 1 ml of blasticidin at 10 mg/ml
pUNO2-mcs:
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ at 100 mg/ml
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