TICAM1-DN

Expression-ready ORF Clones

Alias: TRIF-DN

Toll-like receptor adaptor molecule 1 dominant negative (TICAM1-DN)
TIR domain-containing adapter inducing IFNbeta dominant negative
TIR-containing adaptor molecule 1 dominant negative

TICAM1 (TRIF) is an adapter involved in the MyD88-independent signaling pathway of several TLRs. A dominant-negative form of TICAM1 was shown to completely abolish TLR3-induced signaling and also to block the NF-kB activity mediated by TLR2, TLR4, and TLR7 [1]. Further analysis has revealed that TICAM1 binds preferentially to TLR3 and TLR4 and is essential in their signaling pathways [2]. It predominantly activates IRF-3 and the production of IFN-b rather than NF-kB [3].

Dominant negative TICAM1 (aa387-566) harbors only the TIR domain and lacks the N-terminal and C-terminal portions [1]. Furthermore, it contains a proline to histidine mutation at amino acid 434. This proline residue is conserved among TLRs and is essential for activation of the TLR-mediated signaling.

1. Yamamoto M. et al. 2002. Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-b promoter in the Toll-like receptor signaling. J Immunol, 169(12):6668-6672
2. Yamamoto M. et al., 2003. Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway. Science. 301(5633):640-3.
3. Oshiumi H. et al., 2003. TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction.Nat Immunol. 4(2):161-7.

Specifications

Human TICAM1-DN (pUNO1-hTICAM1-DN)

ORF size : 546 bp
Subclone : NcoI - NheI

pUNO1 Contents

• 20 µg of lyophilized DNA
• 2 x 1 ml blasticidin at 10 mg/ml

Product is shipped at room temperature.

Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.