TLR3-DN

Expression-ready ORF Clones

Alias : TLR3, CD283

TLR3-DN (ΔTIR) : Dominant Negative TIR-less TLR3 gene

TLR3 recognizes double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Stimulation with polyinosine-polycytidylic acid (poly(I:C)), a synthetic analog of dsRNA, was shown to induce hyporesponsiveness in TLR3-deficient mice and marked responsiveness only in 293 cells expressing TLR3 [1], suggesting a specific recognition to poly(I:C) by TLR3.
TLR3 signaling is not elicited by either single-stranded RNA (ssRNA) or dsDNA [2]. TLR3 activation induces cytokine production through a signaling pathway dependent on MyD88. Moreover, poly(I:C) can induce activation of NF-kB and mitogen-activated protein kinases independently of MyD88, and cause dendritic cells to mature [1].
TLR3-DN is a TIR-less form of the TLR3 gene generated by deleting the TIR domain (450 bp). This truncated gene is still able to recognize its ligands but is unable to induce the signaling cascade.

1. Alexopoulou L. et al. (2001). Nature 413(6857):732-8.
2. Matsumoto M. et al. (2002). Biochem Biophys Res Commun. 293(5):1364-9.
3.Takeuchi O.et al. (2001). Int Immunol, 13(7):933-40.