TLR2-DN-HA

Expression-ready ORF Clones

Alias : TLR2, CD282

TLR2-DN (ΔTIR) : Dominant Negative TIR-less TLR2 gene

TLR2 is involved in the recognition of multiple products of Gram-positive bacteria, mycobacteria and yeast. TLR2 has been shown to confer responsiveness to the lipopeptides present in LPS preparations.
However, it seems that some types of LPS can activate TLR2 [1]. TLR2 is known to heterodimerize with other TLRs, a property believed to extend the range of PAMPs that TLR2 can recognize.
TLR2 cooperates with TLR6 in the response to peptidoglycan [2] and diacylated mycoplasmal lipopeptide, and associates with TLR1 to recognize triacylated lipopetides. Furthermore, pathogen recognition by TLR2 is strongly enhanced by CD14.
TLR2-DN is a TIR-less form of the TLR2 gene generated by deleting the TIR domain (429 bp). This truncated gene is still able to recognize its ligands but is unable to induce the signaling cascade. The gene has been fused at the 3'end to the influenza hemagglutinin (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.

1. Netea MG. et al. (2002). Trends Immunol, 23(3):135-9.