pDUO-CD14/TLR2
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDUO-hCD14/TLR2 pDUO bearing human CD14/TLR2 Gene |
Show product |
20 µg |
pduo-hcd14tlr2
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pDUO2-mCD14/TLR2 pDUO2 bearing mouse CD14/TLR2 Gene |
Show product |
20 µg |
pduo2-mcd14tlr2
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pDUO plasmid co-expressing CD14 and TLR2 genes
pDUO-CD14/TLR2 is an expression vector designed to co-express CD14 and TLR2 genes known to interact with each other : human CD14 (1125 bp) and human TLR2 (2352 bp) or murine CD14 (1098 bp) and murine TLR2 (2352 bp).
The genes cloned into pDUO comprise the coding sequence (without introns) from the ATG to the Stop codon.
Back to the topSpecifications
pDUO plasmid with human CD14 and human TLR2 genes.
Name: CD14 / TLR2
Species: Human
Backbone: pDUO
Selection: Blasticidin
pDUO2 plasmid with murine CD14 and murine TLR2 genes.
Name: CD14 / TLR2
Species: Mouse
Backbone: pDUO2
Selection: Hygromycin
Contents
pDUO-hCD14/TLR2:
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
pDUO2-mCD14/TLR2:
- 20 µg of lyophilized DNA
- 1 ml of Hygromycin B Gold (hygromycin) at 100 mg/ml
Description
TLR2 is involved in the recognition of multiple products of Gram-positive bacteria, mycobacteria and yeast. The first studies reported that TLR2 mediated LPS response but TLR2 has since been shown to confer responsiveness to the lipopeptides present in LPS preparations.
However, it seems that some types of LPS can activate TLR2 [1]. TLR2 is known to heterodimerize with other TLRs, a property believed to extend the range of PAMPs that TLR2 can recognize. TLR2 cooperates with TLR6 in the response to peptidoglycan [2] and diacylated mycoplasmal lipopeptide, and assocites with TLR1 to recognize triacylated lipopetides. Furthermore, pathogen recognition by TLR2 is strongly enhanced by CD14.
1. Netea MG. et al. 2002. Trends Immunol, 23(3):135-9.
2. Ozinsky A. et al. 2000. Proc Natl Acad Sci U S A, 97(25):13766-71.
