TIRAP-DN
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pUNO1-hTIRAPb-DN pUNO1 bearing a dominant negative version of the human TIRAP gene |
Show product |
20 µg |
puno1-htirap-dn
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Expression-ready ORF Clones
Alias: MAL, wyatt
TIR domain containing adaptor protein.
MyD88 adapter-like.
Dominant negative TIRAP
TIR domain-containing adaptor protein (TIRAP), also known as Mal, is differentially involved in the signaling to NF-kB by members of the TLR family. TIRAP-deficient mice were shown to respond normally to the TLR3, TLR5, TLR7, and TLR9 ligands, but to be defective in cytokine response to LPS, a ligand for TLR4, as well as ligands for TLR2, TLR1, and TLR6 [1,2]. TIRAP contributes to TLR2 and TLR4 MyD88-dependent signaling but interacts only with TLR4 to activate a MyD88-independent pathway to induce IFN-β and STAT-1-dependent genes [3].
Dominant negative TIRAP (P125H) was generated by mutating the proline at position 125 into a histidine. This TIRAP-DN was shown to inhibit NF-κB activated by LPS-induced TLR4 but not NF-κB activated by IL-1RI or IL-18R suggesting that TIRAP is involved in TLR4 signaling [4].
1. Horng T. et al. 2002. The adaptor molecule TIRAP provides signalling specificity for Toll-like receptors. Nature 420(6913):329-33
2. Yamamoto M. et al. 2002. Essential role for TIRAP in activation of the signalling cascade shared by TLR2 and TLR4. Nature 420(6913):324-9
3. Toshchakov V. et al., 2003. TLR2 and TLR4 agonists stimulate unique repertoires of host resistance genes in murine macrophages: interferon-beta-dependent signaling in TLR4-mediated responses. J Endotoxin Res. 9(3):169-75.
4. Fitzgerald KA et al., 2001. Mal (MyD88-adapter-like) is required for Toll-like receptor-4 signal transduction. Nature. 2001 Sep 6;413(6851):78-83.
Specifications
Human TIRAP-DN (pUNO1-hTIRAPb-DN)
ORF size : 708 bp
Subclone : NcoI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.