ISG Reporter THP-1 Cells

Interferon Regulatory Factor-Inducible Reporter Monocytes

THP1-Lucia™ ISG cells were derived from the human monocytic cell line THP-1, which represents a model of choice to study the activation and signaling of CDSs.

Indeed, THP-1 cells have been shown to express all the CDSs identified so far [1-3], with the exception of DAI [4].

THP1-Lucia™ ISG cells express the secreted luciferase (Lucia) reporter gene under the control of an IRF-inducible promoter. This composite promoter is comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter, which is unresponsive to activators of the NF-kB or AP-1 pathways.

As a result, THP1-Lucia™ ISG cells allow the monitoring of the IRF pathway by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia luciferase in the cell culture supernatant are readily assessed with QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

THP1-Lucia™ ISG cells are resistant to Zeocin®.

THP1-Lucia™ ISG cells are highly responsive to STING and CDS ligands, such as transfected double-stranded nucleic acids.

References:

1. Zhang Z. et al., 2011. The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells. Nat Immunol. 12(10):959-65.
2. Veeranki S. et al., 2011. IFI16 protein mediates the anti-inflammatory actions of the type-I interferons through suppression of activation of caspase-1 by inflammasomes. PLoS One. 6(10):e27040.
3. Arakawa R. et al., 2010. Characterization of LRRFIP1. Biochem Cell Biol. 88(6):899-906.
4. Lippmann J. et al., 2010. IFNbeta responses induced by intracellular bacteria or cytosolic DNA in different human cells do not require ZBP1 (DLM-1/DAI). Cell Microbiol. 10(12):2579-88.