pFUSE-mIgG2Ae1-Fc
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pFUSE-mIgG2Ae1-Fc1
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Show product |
20 µg |
pfc1-mg2ae1
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pFUSE-mIgG2Ae1-Fc2
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Show product |
20 µg |
pfc2-mg2ae1
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pFUSE-mIgG2Ae1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG2 heavy chain mutated L235E + E318A/K320A/K322A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.
pFUSE-mIgG2Ae1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.
Steurer W. et al., 1995. Ex vivo coating of islet cell allografts with murine CTLA4/Fc promotes graft tolerance. J Immunol. 155(3):1165-74.
Back to the topSpecifications
Isotype : IgG2a mouse
Mutations: L235E + E318A/K320A/K322A
Characteristics:
- Reduced binding to FcγRI and C1q
- Reduced ADCC and CDC
Both plasmids are selectable with Zeocin™.
Back to the toppFUSE Contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin® (100 mg/ml)
Product is shipped at room temperature