pDRIVE-SYN1
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
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pDRIVE-Synapsin pDRIVE bearing human SYN1 promoter - LacZ |
Show product |
20 µg |
pdrive-hsynapsin
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pDRIVE5SEAP-Synapsin pDRIVE bearing human SYN1 promoter - SEAP |
Show product |
20 µg |
pdrive5s-hsynapsin
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pDRIVE5Lucia-Synapsin pDRIVE bearing human SYN1 promoter - Lucia |
Show product |
20 µg |
pdrive5lc-hsynapsin
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SYN1 promoter (Neurons) in pDRIVE expression plasmid
Synapsin I is a neuron-specific phosphoprotein that coats the cytoplasmic surface of small synaptic vesicles. The proximal region of the synapsin I promoter (-422 to -22) is sufficient to direct neuron-specific gene expression.
Neuron specificity is conferred by a putative binding site for the zinc finger protein, neuron-restrictive silencer factor/RE-1 silencing transcription factor (NRSF/REST).
The NRSF/REST protein, which is expressed only in non-neuronal cells, represses the activity of constitutive enhancers thus silencing the expression of synapsin I in non-neuronal cells [1].
The synapsin I promoter has been used to achieve highly neuron-specific long-term transgene expression in vivo [2].
References:
1. Schoch S. et al., 1996. Neuron-specific gene expression of synapsin I. Major role of a negative regulatory mechanism. J Biol Chem. 271(6):3317-23.
2. Kugler S. et al., 2003. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. Gene Ther. 10(4):337-47.
Specifications
pDRIVE bearing human SYN1 promoter - LacZ, SEAP or Lucia
Gene Promoter:
Specificity: Neurons
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
