pDRIVE-Desmin
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDRIVE-mDesmin pDRIVE bearing mouse Desmin promoter - LacZ |
Show product |
20 µg |
pdrive-mdesmin
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pDRIVE5SEAP-mDesmin pDRIVE bearing mouse Desmin promoter - SEAP |
Show product |
20 µg |
pdrive5s-mdesmin
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pDRIVE5Lucia-mDesmin pDRIVE bearing mouse Desmin promoter - Lucia |
Show product |
20 µg |
pdrive5lc-mdesmin
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Desmin promoter (Muscle) in pDRIVE expression plasmid
The desmin gene encodes an intermediate filament protein that is present in skeletal, cardiac, and smooth muscle cells.
The first 85 nt upstream of the transcription initiation site of the mouse desmin gene which contain an E box (E1), the binding site of myogenic regulators, are sufficient to confer muscle-specific expression.
High levels of desmin expression are achieved by the addition of an enhancer, located between nt -798 and -976, which contains an additional E box (E2) and a muscle-specific enhancer factor-2 (MEF-2) binding site [1].
This muscle-specific promoter has been used in DNA vaccination experiments to induce the production of antibodies against the hepatitis B surface antigen [2] and the HIV-1 Nef protein [3].
References:
1. Li H., Capetanaki Y. 1994. An E box in the desmin promoter cooperates with the E box and MEF-2 sites of a distal enhancer to direct muscle-specific transcription. EMBO J. 13(15):3580-9.
2. Loirat D. et al., 1999. Muscle-specific expression of hepatitis B surface antigen: no effect on DNA-raised immune responses. Virology. 260(1):74-83.
3. Cazeaux N. et al., 2002. Comparative study of immune responses induced after immunization with plasmids encoding the HIV-1 Nef protein under the control of the CMV-IE or the musclespecific desmin promoter. Vaccine. 20(27-28):3322-31.
Specifications
pDRIVE bearing mouse Desmin promoter - LacZ, SEAP or Lucia
Gene Promoter:
Specificity: Muscle
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
