pDRIVE-SP-B

SP-B promoter (Lung) in pDRIVE expression plasmid

Surfactant protein B (SP-B) is a developmentally and hormonally regulated lung protein that is required for normal surfactant function. SP-B is selectively expressed in bronchiolar and alveolar epithelial cells of the lung.

The human SP-B promoter fragment directs tissue- and lung cell-specific transgene expression [1].

It contains two cis-acting elements for two transcription factors, thyroid transcription factor (TTF)-1 and hepatocyte nuclear factor (HNF)-3 that are important for the specificity and the activation of gene expression in the lung [2].

Promoter activity is strongly inhibited in a dosedependent fashion by phorbol ester, such as 12-O-tetradecanoylphorbol-13-acetate (TPA).

References:

1. Strayer M. et al. 2002. Human surfactant protein B promoter in transgenic mice: temporal, spatial, and stimulus-responsive regulation. Am J Physiol Lung Cell Mol Physiol 282(3):L394-404.
2. Kumar AS. et al., 1997. Phorbol ester down-regulation of lung surfactant protein B gene expression by cytoplasmic trapping of thyroid transcription factor-1 and hepatocyte nuclear factor 3. J Biol Chem. 72(33):20764-73.