pDRIVE-CD43

CD43 promoter (Leukocytes & platelets) in pDRIVE expression plasmid

CD43, a major sialoglycoprotein expressed on the surface of leukocytes and platelets, plays an important role both in adhesion and signal transduction.

The CD43 promoter does not contain any TATA or CAAT boxes, but is highly enriched in G and C nucleotides and contains short repeat sequences similar to those found in promoters of "housekeeping" genes [1].

Transcription from the CD43 promoter is mediated by the transcription factor Sp1, which binds to repeats of a GGGTGG motif in the 5'-flanking sequence.

This regulatory region is ubiquitously functional in mammalian cells, providing a high transcriptional potential [2].

References:

1. Shelley CS. et al. 1990. Structure of the human sialophorin (CD43) gene. Identification of features atypical of genes encoding integral membrane proteins. Biochem J. 270(3):569-76.
2. Kudo S. & Fukuda M. 1995. Tissue-specific transcriptional regulation of human leukosialin (CD43) gene is achieved by DNA methylation. Biol Chem 270(22):13298-302.